DNA Sequence Analyzer
Paste any DNA sequence for instant bioinformatics analysis — GC content, ORF finder, protein translation, restriction enzymes, primer design, and mutation detection.
Paste a DNA sequence above and click Analyze to see statistics.
Run the ORF finder above.
Configure options above and click Translate.
Paste two sequences above to compare them.
Run the restriction enzyme finder above.
Configure primer parameters and click Design Primers.
Sequence Statistics
Length, GC/AT content, nucleotide counts, molecular weight, and reverse complement in one click.
ORF Finder
Scan all 6 reading frames for ATG–stop codon open reading frames with protein length estimates.
Protein Translator
Translate DNA to amino acids using the standard genetic code with codon-by-codon display.
Mutation Detector
Compare two sequences to find substitutions, insertions, and deletions with position annotations.
Restriction Enzymes
Find cut sites for 30+ common restriction enzymes including EcoRI, BamHI, HindIII, and more.
Primer Designer
Design forward and reverse primers with Tm calculation, GC%, and GC clamp analysis.
How to Use the DNA Sequence Analyzer
Paste any raw DNA sequence (A, T, G, C characters — spaces, numbers and newlines are automatically stripped) into the input box and click Analyze Sequence. All six analysis tools will receive your sequence. Switch between tabs to explore different analyses.
What sequences can I analyze?
Any DNA sequence in IUPAC format. The tool automatically removes whitespace, numbers, and FASTA header lines (lines starting with >). Sequences from GenBank, Ensembl, NCBI, or your own lab data all work.
GC Content Calculator
GC content is the percentage of guanine and cytosine bases in a DNA sequence. It affects melting temperature, genome stability, and codon usage bias. Our calculator shows exact nucleotide counts, GC%, AT%, and a visual composition bar.
- GC content above 50% → more thermally stable DNA
- Human genome average: ~41% GC content
- Bacterial genomes range from ~25% (Mycoplasma) to ~72% (Streptomyces)
Open Reading Frame (ORF) Finder
An open reading frame is a sequence of DNA that begins with a start codon (ATG) and ends with a stop codon (TAA, TAG, or TGA) without any intervening stop codons. ORFs are the primary candidates for protein-coding genes.
6-Frame Translation
DNA can be read in six frames: three on the forward strand (+1, +2, +3) and three on the reverse complement strand (−1, −2, −3). This tool scans all six frames and reports every ORF above your minimum length threshold.
DNA to Protein Translation
DNA is transcribed to mRNA and then translated to protein via the genetic code. Each three-nucleotide codon encodes one of 20 amino acids. This translator uses the standard genetic code (NCBI translation table 1).
Restriction Enzyme Sites
Restriction enzymes cut DNA at specific recognition sequences. Our finder scans for 30+ common enzymes used in molecular cloning including:
- EcoRI — GAATTC (most common cloning enzyme)
- BamHI — GGATCC (compatible with BclI ends)
- HindIII — AAGCTT (useful for lambda phage cloning)
- NotI — GCGGCCGC (8-cutter, rare cutter)